Livestock Research for Rural Development 31 (2) 2019 Guide for preparation of papers LRRD Newsletter

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Effect of dietary Turmeric (Curcuma longa) extract powder on productive performance and egg quality of black-bone chicken (Ac chicken)

Truong Van Phuoc, Nguyen Nhut Xuan Dung1, Luu Huu Manh1 and Nguyen Nhut Vinh Tu2

Tien Giang University, Cantho, Vietnam
truongvanphuoc@tgu.edu.vn
1 Cantho University, Cantho, Vietnam
2 Nong Lam University, Ho Chi Minh City, Vietnam

Abstract

A study was conducted to evaluate the effect of dietary Turmeric extract powder supplementation on egg production, feed intake, feed conversion ratio and egg quality of Ac laying hens from 26 to 34 and 50 to 58 weeks of age. The experiment consisted of two treatments: BS: basal diet (control, no turmeric powder); TP: (0.1% turmeric powder) with ten replicates.

During 26-34 weeks of age, turmeric addition increased egg production and egg mass and improved feed conversion. From 50-58 weeks of age, the addition of turmeric powder increased feed intake and body weight but had no effect on egg mass production. Dietary turmeric treatments did not influence egg quality.

Keywords: curcumin, egg mass, egg weight, feed conversion


Introduction

Ac chickens are a local black-bone chicken breed that originated in the Mekong delta and are commonly kept in rural areas. They have different characteristics in appearance and size; the body is covered with white feathers, while the beak, tongue, skin, meat and bones are black; their legs are characterized by five black toes. They are a low-growing breed and have small body size with mature weight of 0.9 kg. Ac hens have earlier age at first lay (105-112 days), then slowly reach the peak at 25 to 40 weeks and quickly decline in egg rate after 50 weeks of age as compared with the other commercial breeds. Ac hens are normally culled after 60 weeks of age due to low egg production and high demand of black boned chicken meat. Ac chickens are dual-purpose birds and kept for meat and eggs. Although low in growth performance, their meat and eggs have outstanding flavour. Ac chicken meat is famous with medicinal and health-promoting effects and used as a healthy food for sickness, pregnant women, old people and children. People believe that black meat is functional and has special nutritive value. Eggs of Ac chicken are small size (29-32 g (Tran Thi Mai Phuong and Nguyen Van Thien et al 2008), high in yolk/white ratio of 61-64% (Truong van Phuoc and Nguyen Van Thieet al 2018). Their eggs are palatable and suitable for children. The demand of meat and eggs has been increasing, and because of their relatively high prices, the population of Ac chicken is rising. In the backyard scavenging system, egg production of Ac chicken is low (90 - 95 eggs/year (Tran Thi Mai Phuong and Nguyen Van Thien 2008). However, it can be improved up to 180 – 190 eggs/year by providing good nutrition and disease control (Truong Van Phuoc et al 2016). Because of small size, low growth and egg rate, Ac chickens are mainly keeping in small scale, units, easy to access with low investment. They are a major source of income and nutritionally rich food for households.

The use of antimicrobial growth promoters to improve performance in livestock and chicken has led to the emergence of antibiotic-resistant strains of microbes of risk to human health. Antimicrobial growth promoters have been banned in the European Union (EU 2005) and in USA (FDA 2016). Thus, there is a need to find and develop safer alternative sources to antibiotics for livestock and chickens in particular.

There are many studies done on using of herbal extracts as natural antioxidants (Osawa et al 1995), growth promoters and for disease prevention (Petrovic et al 2012; Abou-Elkhair 2014; Alzawqari et al 2016). Among them, turmeric (Curcuma longa L.), a culinary spice, contains many compounds with high biological activities as anti-microbials (Araujo and Leon 2001; Kim et al 2013), anti-coagulants, anti-diabetics and anti-ulcers (Lokova et al 2001), anti-inflammation, antioxidant (Gandhi et al 2011). Platel and Srinivasan (1996, 2000) reported that curcumin extracted from turmeric stimulates bile acid secretion, and increases lipase, amylase and protease activities. Curcumin can improve liver function, reduce plasma triglycerides, and reduce LDL cholesterol (Emadi and Kermanshahi 2007; Seo et al 2008; Gandhi et al 2011).

The aim of the present study was to evaluate the effects of turmeric extract as a feed additive on feed intake, reproduction, feed conversion and egg quality of Ac laying hens during the peak and past their peak of egg production period.


Materials and methods

Location and duration

This study was conducted at Phuoc Khang farm in My Tho city, Tien Giang province, Vietnam from February 2017 to May 2017.

Birds and housing

Two hundred and eighty Ac hens were studied in two periods, based on their reproductive characteristics; from 26 to 34 weeks of age which is the peak of lay and 50 to 58 weeks which is a period of low egg production.

Each treatment was randomly allotted to 20 cages with 7 hens per cage in an open-side house (Photos 1 and 2), ventilated with fans and with a daily 17L:7D lighting regime . All birds were vaccinated according to the provincial veterinary program. Feed and water were provided ad libitum.

Photo 1. Housing and experimental Ac laying hens
Experimental design and diets

The experiment was arranged as a completely randomized design of two treatments with ten replicates of seven hens in each. The trial lasted for 8 weeks. The treatments were:

BS: basal diet (Table 1) with no turmeric extract powder (control)

TP: basal diet + 2 g turmeric extract powder (containing 5% curcumin)/kg feed

Turmeric powder was extracted from rhizomes with alcohol (purity of 5% curcumin; 2.53% moisture), purchased from Kim Tai CO., Ltd, HCM City.

Table 1. Feed formulation and composition of basal diet (air-dry basis)

Ingredients

(%)

Maize

49

Rice bran

12

Fish meal

3

Soybean meal

24

Tra fish oil

1

Coarse limestone

4

Fine limestone

4

Nutrimix – Layer1

3

Composition

Dry matter

89.9

Ash

12.7

Crude protein

17.4

Ether extract

4.93

Crude fibre

2.48

Calcium

4.31

Phosphorus

0.71

Lysine

1.12

Methionine

0.44

MEn (MJ/kg)

11.8

(1) Supplied per kg diet: Fe (200mg), Cu (40mg), Zn (60mg), Mn (60mg), Co (0.3mg), Iodine (0.3mg), Se (0.3mg) vitamin A (8000 IU), vitamin B6 (3mg), vitamin D3 (2500 IU), vitamin B12 (15mcg), vitamin E (30mg), pantothenic acid (8mg), vitamin B1 (1.5mg), folic acid (0.5mg), vitamin B2 (4mg), biotin (100 mcg), vitamin K3 (2mg), niacin (20mg), vitamin C (100mg), choline chloride (500mg). (2) Amino acid composition was analyzed from Upscience laboratory. (3)MEn was calculated according to Truong Van Phuoc et al. (2016b)

Measurements

Live weigh gain or loss was calculated from weight at beginning and the end of the experiment. Egg number was recorded daily to calculate egg production as a percentage of eggs produced per bird. Egg weight was determined by weighing all eggs produced per experimental unit by a digital balance to the nearest of 0.01 g accuracy. Feed intake was recorded by feeding weighed quantities of feed daily and subtracting the left over from the previous day. Egg mass was calculated as egg production*egg weight. Feed conversion (g/g) was obtained from feed intake/egg mass. Six eggs were collected from each experimental unit during the last 3 consecutive days at the end of each period and individually weighed. Shape index, egg shell thickness, yolk and albumin index were done according to Anderson et al (2004). Haugh unit (HU) was calculated as follows:

HU=100 log (H+7.57−1.7W37) (Haugh 1937),

where, H is albumen height (mm), measured by spherometer and W is egg observed weight (g). Egg yolk color was evaluated by using Roche colorimetric fan (Yolk color fan, Roches, Switzerland).

Statistical analysis

Data were subjected to analysis of variance (ANOVA) using the General Linear Model (GLM) available in Minitab 13.2. The Tukey test in the same software was used to detect differences among treatment means.


Results

26 – 34 weeks of age

Egg production tended to increase in hens supplemented with turmeric (58.5% vs 54.4%, p= 0.08; Table 2) but the additive had no influence on egg weight. Turmeric supplementation did not affect the feed intake; feed conversion ratio was better (lower) for the hens supplemented with turmeric.

There was a minor increase in weight of hens fed the TU diet, but no change in hens fed the control diet. Addition of Turmeric powder did not influence egg shape index, Haugh unit, yolk and albumen index, egg shell thickness, yolk and albumen ratio (Table 3).

50-58 weeks of age

Turmeric supplementation increased feed and egg weight but did not influence egg production nor feed conversion. There were minor losses in body weight in hens fed the control diet (-7.85 g), on the other hand, there was gain in weight in hens supplemented with turmeric (7.14 g).

There were no differences in egg quality (shape index, Haugh unit, and egg components) between the treatments.

Table 2. Effects of dietary treatments on hen performance

Control

TU

SEM

p value

26-34 week of age

Daily feed intake, g

58.5

57.8

0.28

0.11

Egg production, %

54.4

58.5

1.56

0.08

Egg weight, g

35.7

36.4

0.38

0.22

Feed conversion, g/g

3.06

2.74

0.07

0.01

Egg mass, g/hen/day

19.4

21.3

0.52

0.02

Body weight change, g

0.00

10.00

-

-

50-58 week of age

Daily feed intake, g

50.5

52.8

0.57

0.01

Egg production, %

44.1

44.4

1.89

0.93

Egg weight, g

37.8

39.3

0.40

0.01

Feed conversion, g/g

3.08

3.08

0.13

0.90

Egg mass, g/hen/day

16.7

17.4

0.74

0.46

Body weight change, g

-7.85

7.14

-

-



Table 3. Effects of dietary treatments on egg characteristics

Control

TU

SEM

p value

26-34 weeks of age

Shape index

77.3

78.7

1.01

0.29

Haugh unit

77.4

78.4

1.87

0.57

Yolk index

0.46

0.48

0.02

0.56

Albumin index

0.07

0.08

0.01

0.12

Yolk ratio, %)

35.01

34.1

1.11

0.63

Albumin ratio, %)

51.3

52.6

1.21

0.34

Shell ratio, %)

13.7

13.3

0.26

0.59

Shell thickness, mm)

0.34

0.35

0.01

0.45

Yolk color

6.15

6.00

0.22

0.23

50-58 weeks of age

Shape index

78.1

78.6

0.96

0.67

Haugh unit

79.3

81.9

2.16

0.39

Yolk index

0.46

0.46

0.01

0.80

Albumin index

0.06

0.06

0.01

0.47

Yolk ratio (%)

35.3

36.3

1.04

0.50

Albumin ratio (%)

51.1

50.2

1.23

0.62

Shell ratio (%)

13.6

13.5

0.50

0.86

Shell thickness (mm)

0.31

0.32

0.01

0.83

Yolk color

6.60

6.60

0.19

0.71


Discussion

The evidence for improved hen performance from turmeric supplementation is indicated by:

From 26 to 34 weeks: the tendency for an increase in egg mass (by10%), the 10% better feed to egg conversion and the 10 g gain in body weight versus no change in the control

From 50 to 58 weeks: no change in egg mass nor in feed conversion but an increase in body weight versus a loss in the control treatment.

In the later stage of the laying cycle (50-58 week of age period), the laying potential of the Ac breed is known to decline. So an egg laying response to turmeric was not unexpected; however, the turmeric group experienced a gain in weight while the control group lost weight – implying a better nutritional status due to the turmeric powder additive in this period.

The benefits from turmeric powder supplementation on egg production in the 28-34 weeks of age stage are supported by mostly positive results in the literature (Malekizadeh et al 2012; Park et al 2012; Rahardja et al 2015; Kanagaraju et al 2017; Gumus et al 2018; Johannah et al 2018).

Reports of turmeric powder additives on hen live weight indicated no benefits (Gumus et al 2018), However, these authors used “specialized” layer breeds which can be expected to favour egg production rather than body weight gain. By contrast, the Ac breed is considered to be of dual-purpose characteristics as witnessed by its capacity to produce meat that is much appreciated by consumers (eg:…. the black boned chicken meat has more flavour and has a high price!!).

This present study found that turmeric supplementation did not influence egg quality especially egg yolk color that is an important feature highly preferred by consumers. This finding is in agreement with those of Laganá et al (2011) and da Silva et al (2018), who reported that dried turmeric root did not promote egg yolk pigment. In contrast, Gumus et al (2018) and Park et al (2012) reported that yolk color in eggs from hens fed 0.5% turmeric powder increased in comparison with the control.

Chicken farmers often add synthetic pigments to the feed to improve egg yolk color, but this also increases cost. Thus, there is increasing interest in finding natural sources of pigment. Turmeric could be a promising alternative material; however, results are variable and further studies are needed.


Conclusion


References

Abou-Elkhair R, Ahmed H A and Selim S 2014 Effects of black pepper (piper nigrum), turmeric powder (curcuma longa) and coriander seeds (coriandrum sativum) and their combinations as feed additives on growth performance, carcass traits, some blood parameters and humoral immune response of broiler chickens.Asian-Australas Journal of Animal Science 2014 Jun; 27(6):847-54.

Alzawqari M H, Al-Baddany A A, Al-Baadani H H, Alhidary I A, Khan R U, Aqil G M, Abdurab A 2016 Effect of feeding dried sweet orange (Citrus sinensis) peel and lemon grass (Cymbopogon citratus) leaves on growth performance, carcass traits, serum metabolites and antioxidant status in broiler during the finisher phase. Environmental Science Pollution Research 23(17):17077-82. Epub 2016 May 21.

Anderson K E, Tharrington J B, Curtis P A, and Jones F T 2004 Shell characteristics of eggs from historic strains of Single Comb White Leghorn chickens and the relationship of egg shape to shell strength. International Journal of Poultry Science 3:17–19.

Araujo C A C and Leon L L 2001 Biological activities of Curcuma longa L. Memorias do Instituto Oswaldo Cruz, 96: 723-728. 2001.

Bartov I and Bornstein S 1980 Studies on egg yolk pigmentation: Effect of ethoxiquin on xanthophylls within and among genetic sources Poultry Science 50 1460-1461

Da Silva W J, Gouveia A B V S , de Sousa F E , dos Santosa F R, Minafra-Rezende  C S, Silva J M S  and Minafra C S 2018 Turmeric and sorghum for egg-laying quails. Italian Journal of Animal 17:2, 368-376.

Emadi M and Kermanshahi H 2007 Effect of turmeric rhizome powder on the activity of some blood enzymes in broiler chickens. International Journal of Poultry Science, 6: 48-51.

EU 2005 Ban on antibiotics as growth promoters in animal feed enters into effect. http://europa.eu/rapid/press-release_IP-05-1687_en.htm. Brussels; 2005

FDA 2016 Center for Veterinary Medicine, FDA Reminds Retail Establishments of Upcoming Changes to the Use of Antibiotics in Food Animals, https://www.fda.gov/AnimalVeterinary/NewsEvents/CVMUpdates/ucm507355.htm Rockville, Maryland, 2016. ]

Gandhi P, Khan K and Chakraverty N 2011 Soluble curcumin: a promising oral supplement for health management. Journal of Applied Pharmaceutical Science, 1: 1-7.

Gumus H, Oguz M N , Bugdayci K E , Oguz F K 2018 Effects of sumac and turmeric as feed additives on performance, egg quality traits, and blood parameters of laying hens. Revista Brasileira de Zootecnia 47: e2017 0114, 2018

Hassan S M 2016 Effects of adding different dietary levels of turmeric (Curcuma longa Linn) powder on productive performance and egg quality of laying hens. International Journal of Poultry Science, 15: 156-160.

Haugh R R 1937 The Haugh unit for measuring egg quality. U.S. Egg Poultry Magazine. 1937;43:552–555.

Johannah N M, Joseph J, Maliakel A B and Krishnacuma IM 2018 Dietary addition of a standardized extract of turmeric (TurmaFEED TM) improves growth performance and carcass quality of broilers. Journal of Animal Science and Technology, 60, 8. http://doi.org/10.1186/s40781-018-0167-7

Kanagaraju P, Omprakash AV, Rathnapraba S and Rajmanohar G 2017 Effect of Turmeric (Curcuma Longa) on the Egg Production and Bio-Chemical Parameters in Layers Indian Veteranary Journal, 94 (04) : 24 - 26

Kim D K, Lillehoj H S, Lee S H, Jang I S, Lillehoj E P, Bravo D 2013 Dietary Curcuma longa enhances resistance against Eimeria maxima and Eimeria tenella infections in chickens, Poultry Science, Volume 92 (10) 2635–2643, https://doi.org/10.3382/ps.2013-03095

Laganá C, Pizzolante C C, Saldanha E S and de Moraes J E 2011 Turmeric root and annato seed in second-cycle layer diets: performance and egg quality. Revista Brasileira de Ciência Avícola. 13(3):171-6.

Lokova M Y, Buzuk G N, Sokolova S M and Kliment-eva N I 2001 Chemical features of medicinal plants. Applied Biochemistry and Microbiology, 37: 229-237.

Malekizadeh M, Moeini M M and Ghazi S H 2012 The effects of different levels of ginger (Zingiber officinale Rosc) and turmeric (Curcuma longa Linn) rhizomes powder on some blood metabolites and production performance characteristics of laying hens. Journal Agricultural Science Technology 14:127-134.

Osawa T, Sugiyama Y, Inayoshi M and Kawakisi S 1995 Antioxidative activity of tetrahydrocurcuminoids. Bioscience, Biotechnology, and Biochemistry 59:1609-1611

Park S S, Kim J M, Kim E J, Kim, Kim H S B and Kang C W 2012 Effects of dietary turmeric powder on laying performance and egg qualities in laying hens. Korean Journal Poultry Science 39: 27-32.

Petrovic V, Marcincak S, Popelka P, Simkova J, Martonova M, Buleca J, Marcincakova D,Tuckova M, Molnar L and Kovac G J 2012 The effect of supplementation of clove and agrimony or clove and lemon balm on growth performance, antioxidant status and selected indices of lipid profile of broiler chickens. Animal Physiology Animal Nutrition (Berl). 96(6):970-977.

Platel K and Srinivasan K 2000 Influence of dietary spices and their active principles on pancreatic digestive enzymes in albino rats. Nahrung, 44: 42-46. 2000

Platel K, Srinivasan K 1996 Influence of dietary spices or their active principles on digestive enzymes of small intestinal mucosa in rats. International Journal of Food Science Nutrition, 47(1):55- 59.

Rahardja D P, Hakim M R and Lestari V S 2015 Egg production performance of old laying henfed dietary turmeric powder. International Journal of Biological Biomolecular Agriculture and Food Biotechnological Engineering 9:717-721

Seo K, Choi M, Jung U J, Kim H J, Yeo J, Jeon S M and Lee M K 2008 Effect of curcumin supplementation on blood glucose, plasma insulin, and glucose homeostasis related enzyme activities in diabetic db/db mice. Molecular Nutrition and Food Research, 52: 995-1004

Tran Thi Mai Phuong and Nguyen Van Thien 2008 Ac Chicken Production in Vietnam. 7th RBI Global Conference on the Conversion of Animal Genetic Resources “Impact of the Globalisation on the Animal Genetic Resources” Hanoi, Vietnam, 14-18 September, 2008.

Truong Van Phuoc, Nguyen N X D and Luu H M 2016 Effect of Protein and Energy Levels on performance and egg quality of black-boned layers (Ac chicken) at early first production cycle. Tropical Animal Science and Production Conference 2016 Thailand (TASP 2016), July 26-29, 2016. p 209-213.

Truong Van Phuoc, Nguyen N X D and Luu H M 2018 Effects of dietary lysine to energy ratios on reproductive performance, egg quality, nitrogen utilization and blood parameters of Ac Layers. Journal of Agricultural Science and Rural Development. (10) 94-103


Received 26 November 2018; Accepted 17 January 2019; Published 1 February 2019

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