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Citation of this paper

Effect on performance of Hararge Highland sheep of feeding “Kocho” waste as part of the concentrate supplement

Kochito Wolde, Mengistu Urge1 and Meseret Girma1

Department of Animal Sciences, Mizan Tepi University, P O Box 260, Mizan Tepi, Ethiopia
1 School of Animal and Range Sciences, Haramaya University, P O Box 138, Dire Dawa, Ethiopia
meseretgirma4@gmail.com

Abstract

A study was conducted to evaluate the effect of feeding Kocho waste (KW) as a substitute for part of the concentrate mixture (a 2:1 ratio of maize and wheat bran) on growth performance of Hararghe Highland sheep. Twenty-four yearling intact male sheep with initial body weights of 16.3±2.08 kg were assigned to four dietary treatments in a completely randomized block design. The treatments were: replacement levels of ECM of 0, 25, 50 and 75% by kocho waste, the other components of the diet being noug seed cake and natural grass hay.

Over a 90 day growth period, there were positive responses in live weight gain and feed conversion to inclusion of kocho waste at the 20% level of the diet. However, when the kocho waste reached 30% of the diet DM, growth rate and feed efficiency became worse. It is postulated that at low levels, kocho waste provides benefits due to the prebiotic effect resulting from its content of autolyzed yeast cells but at high levels this did not compensate for the superior value of maize as source of glucose precursors, which are a major determinant of growth rate and feed efficiency in ruminant animals.

Key words: Ensete ventricosum, fermentation, prebiotics


Introduction

The Enset plant (Ensete ventricosum), known as “false banana” , is widely grown in southern and southwestern parts of Ethiopia (Admasu 2002). Different parts of Enset are used as feed for livestock (Brandt et al 1997; Mohammed et al 2013). One of these products is “Kocho” produced by scraping the starchy pulp from the Enset stem and fermenting it in a pit in the ground (Photo 1; Admasu 2002). The product of the fermentation is primarily used for human consumption. In 2017, from 123,479,334.00 enset plants 3,162,563 tonnes of kocho were produced (CSA 2017). However, the upper layers of the fermented mass are usually discarded due to discoloration. This component which is not suitable for human consumption is traditionally fed to livestock.

Photo 1. Ensiling “Kocho” in a “pit” about 1.2m deep

The objective of the present study was to evaluate Kocho waste (Dupo) as a partial replacement for the concentrate (a mixture of maize, wheat bran and noug seed cake) used to supplement grass hay for fattening sheep.


Materials and methods

Starter culture for Kocho

The corms (Photo 2) of selected Enset plants are traditionally used as substrate for the preparation of a starter culture (gamancho). The corm is pulverized with a serrated and sharp-edged tool made from animal bone (scapula) to make it ready for fermentation. The prepared corms are then wrapped with fresh enset leaves and left at ambient temperature for 8 days. On the 5th day, the ground corm is exposed to the sun for 5 to 12 hours and again wrapped with fresh enset leaves and allowed to ferment for 3 to 5 days more (Tiruha et al 2014).

Preparing Kocho

Mature enset plants are selected chopped and the starchy internal part pounded into “dough”. The fermented starter culture is mixed with the fresh dough and left to ferment in a pit for 3-5 days or more (Tiruha et al 2014).

Experimental animals

The experiment was conducted at Haramaya University sheep farm, Ethiopia. Twenty-four intact male yearling Hararge highland sheep were purchased from a local market. They were quarantined for 3 weeks and treated against internal and external parasites. They were adapted to the experimental diets for 15 days before data collection.

Feed preparation

The Kocho waste was collected from house-holders in Keffa and Bench Maji zones of SNNPR. It was sun-dried by spreading over a clean mat until it became safe for storage.

Photo 2. The Enset plant in the natural state (A) and shown diagrammatically (B)
Experimental design and treatments

The experiment was conducted according to a randomized block design with four treatments and six replicates. The sheep were blocked based on their initial body weight and allocated to 4 treatments within each block. The treatments consisted of increasing levels of Kocho waste ( Dupo) replacing the energy concentrate at 4 levels: 0, 25, 50 and 75% on DM basis (Table 1). The energy concentrate (ECM), kocho waste (KW) and noug cake (NSK) were mixed together and fed ad libitum along with natural grass hay also ad libitum. All animals had free access to water.

Table 1. Details of the feeding system

Treatments

Grass hay

Concentrate supplement, % as DM

KW

ECM

NSC

75ECM#

Ad libitum

0

75

25

50ECM+25KW

Ad libitum

25

50

25

25ECM+50KW

Ad libitum

50

25

25

25ECM+75KW

Ad libitum

75

0

25

#67% maize; 33% wheat bran

Digestibility measurement

This was for 7 days after the 90-day feeding trial when the sheep were harnessed with fecal collection bags for total fecal collection. Representative samples of feces were dried at 60°C for 72 hours prior to analysis.

Growth performance and feed conversion

Live weights were taken every 10 days in the morning before the daily feed offer. Live weight gains were estimated from the linear regression of liveweight (Y) at 10-day interval s (X). Feed conversion was calculated by dividing ADG (g/day) by DMI (g/day).

Chemical Analysis

The chemical analysis of feeds and feces followed AOAC (1990) procedures. Neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) were determined by procedures in reported by Van Soest and Robertson (1985).

Statistical Analysis

The final data were subjected to analysis of variance in a randomized complete block design using the general linear model procedure of the ANOVA program in the software of SAS (2002). The model for ANOVA analysis was: Yij= µ + ti + bj + eij

Yij= the response variable; µ= overall mean; ti = treatment effect (feed); bj = block effect (initial body weight) and eij = random error


Results

Chemical composition of feeds

The crude protein content of Kocho waste (Dupo) was 7.1% (in DM) and lower than in maize (Table 2). Temesgen (2013) reported lower values of 4.42 - 5.09% of crude protein in Kocho waste. The NDF and ADF in Kocho waste were lower than in maize.

Table 2. Composition of ingredients, concentrate and hay

DM%

Chemical composition (% in DM)

OM

CP

NDF

ADF

ADL

Ash

Natural hay

90

81.38

8.64

76

45

6.56

8.62

KW (Dupo)

92

90.1

7.08

30

4.3

3.85

1.9

Wheat bran

90

85.8

17.02

43

11.72

3.6

4.2

Noug cake

92

82.37

36.9

40

23

8.5

9.63

Maize

90

88.23i

8.04

38

5.2

3.6

1.77

ADF=acid detergent fiber; CP=crude protein; DM=dry matter; NDF=neutral detergent fiber

Feed intake , live weight gain and feed efficiency

Feed intake and DM digestibility did not vary among treatments (Table 3). Live weight gain and feed conversion efficiency showed curvilinear responses to inclusion level of kocho waste in the diet (Figures 1 and 2) with improvements in both criteria with up to 20% kocho waste in the diet but with no further improvement when the kocho waste reached 31% of the diet DM.

Table 3. Mean values for DM intake, digestibility and growth performance of Hararge highland sheep fed natural hay supplemented with different proportions of kocho waste as replacement for maize and wheat bran

KW, % in diet DM

SEM

p

0

10.4

20.4

31.1

DM intake, g/d

Hay

447

447

460

440

Supplement

316

318

320

322

Total intake

763

765

780

772

DM digestibility, %

68.4

71.5

73.6

75.6

1.078

0.244

Initial weight, kg

23.3c

24.34bc

27.95a

26.5ab

0.82

0.007

Final weight, kg

83.3c

87.88bc

127.22a

111ab

7.99

0.005

LW gain, g/d

86.0 a

92.1 a

130 b

129 b

7.97

0.007

FCE#

0.109b

0.11b

0.16a

0.14ab

0.011

0.01

a, b, means within rows without common superscript are different at p<0.05       #LW gain/DM intake



Figure 1. Relationship between live weight gain and proportion of
the maize-wheat bran concentrate replaced by kocho waste
Figure 2. Relationship between feed conversion efficiency and proportion
of the maize-wheat bran concentrate replaced by kocho waste

The performance response curves observed in this experiment bear a close resemblance to the responses observed in a previous experiment in which a byproduct (Atella) from local brewing of sorghum grain was fed to sheep (Ayfokir et al 2020). In that instance the initial response to the supplement was attributed to a prebiotic effect induced by the products (β-glucan and related products) derived from the autolysis of the residual yeast cells at the end of the brewing process. The subsequent decline in animal performance with more than 30% replacement of the concentrate by Atella was attributed to the decline in availability of essential nutrients (glucose precursors and amino-acids) as the Atella replaced the nutrient-rich concentrate. We propose that a similar relationship existed between the Kocho waste and the maize grain-wheat bran part of the concentrate that it replaced. Thuy Hang et al (2018) reported a similar curvilinear response to brewers’ spent grain fed at 0, 2, 4 and 6% the diet of growing goats. The maximum response in growth rate was with 4% of the supplement with a major decrease in response as the level of the supplement was raised to 6% (Figure 3).

Figure 3. Curvilinear relationship between growth rate of goats and level
of brewers’ grains in the diet (Thuy Hang et al 2018)

The inflexion point for any given supplement thought to provide “prebiotic” properties will be determined by the concentration in the supplement of the compounds responsible for the ”prebiotic” effect. In ruminants this research area is still poorly understood.


Conclusions

Live weight gain and feed conversion efficiency showed curvilinear responses to inclusion level of kocho waste in the diet with:


References

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